Erythrocyte Ankyrin anticorps

N° du produit ABIN2462605

Cet anticorps Lapin Polyclonal détecte spécifiquement Erythrocyte Ankyrin dans WB, IHC et ELISA. Il présente une réactivité envers Humain, Souris, Rat et Chien.

Aperçu rapide pour Erythrocyte Ankyrin anticorps (ABIN2462605)

Antigène: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Erythrocyte Ankyrin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-Erythrocyte Ankyrin anticorps

Purification: Antibody is purified by protein A chromatography method.

Immunogène: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANK1.

Information d'application

Indications d'application: ANK1 antibody can be used for detection of ANK1 by ELISA at 1:312500. ANK1 antibody can be used for detection of ANK1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Conseil sur la manipulation: As with any antibody avoid repeat freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ANK1 antibody at -20 °C.

Détails sur Erythrocyte Ankyrin

Antigène: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Autre désignation: ANK1

Sujet: Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known.Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats, a central region with a highly conserved spectrin binding domain, and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described, however, the precise functions of the various isoforms are not known. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.

Poids moléculaire: 17 kDa, 206 kDa, 15 kDa, 8 kDa

ID gène: 286

NCBI Accession: NP_065211

UniProt: Q53ER1

Pathways: Synaptic Membrane